Culture of Single Cells - Single cells can be cultured using the following techniques:
(1) filter paper raft-nurse tissue technique,
(2) microchamber technique,
(3) microdrop method,
(4) Bergman's plating technique and
(5) thin layer liquid medium.
Isolated single cells fail to divide in normal tissue culture media. Therefore, either a nurse tissue or a conditioned medium is used for their culture. A conditioned medium is a medium in which plant cells have been grown for about 48 hr (cells are then filtered out).