Culture tubes and flasks are usually stoppered with cotton plugs, which are often wrapped in cheese cloth, but preparing such plugs on a large scale may be time consuming and inconvenient. Many workers use caps made of either polypropylene or a metal (aluminium/stainless steel) for this purpose; these caps have proved quite effective but their design may considerably affect their performance.
Culture vessels and other labware are generally soaked in a suitable nontoxic detergent solution overnight, washed with a suitable brush, thoroughly rinsed clean with tap water, followed by a rinse with distilled water and dried in a hot air oven (75-80°C). Culture vessels having contamination or some dried agar sticking to the wall are first autoclaved. Washed culture vessels should be stored preferably in a dust-proof cupboard or cabinet.
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