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Culture Vessels and their Washing - Generally, glass culture vessels are used as they are cheaper, reusable and autoclavable. It is desirable to use only borosilicate or Pyrex glass wares as ordinary soda glass may be toxic to some tissues.
Culture vessels made of clear plastic are available for a variety of purposes; these vessels are generally presterilized and disposable but certain types are autoclavable and, therefore, reusable. In general, plastic vessels are, in the long run, much costlier than glass vessels.
Tissues are generally cultured in culture tubes (rimless tubes of 25 x 150 mm or larger), flasks (long neck or even ordinary conical flasks) and Petri plates; but many especially designed dishes are also used. Wide mouth bottles, including milk bottles are often employed especially for micropropagation work. Suspension cultures, of necessity, are maintained in long neck culture flasks.

Culture tubes and flasks are usually stoppered with cotton plugs, which are often wrapped in cheese cloth, but preparing such plugs on a large scale may be time consuming and inconvenient. Many workers use caps made of either polypropylene or a metal (aluminium/stainless steel) for this purpose; these caps have proved quite effective but their design may considerably affect their performance.
Culture vessels and other labware are generally soaked in a suitable nontoxic detergent solution overnight, washed with a suitable brush, thoroughly rinsed clean with tap water, followed by a rinse with distilled water and dried in a hot air oven (75-80°C). Culture vessels having contamination or some dried agar sticking to the wall are first autoclaved. Washed culture vessels should be stored preferably in a dust-proof cupboard or cabinet.