Estimation of Cell Growth,Haemocytometer,Suspension Cultures,Callus Cultures,Cell Mass

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	Estimation of Cell Growth - Cell number is the most informative measure of cell growth. This measurement is applicable to only suspension cultures, and even there cell aggregates must be treated, e.g., with pectinase, to dissociate them into single cells before counting the cell number in a haemocytometer. Therefore, cell number is estimated only where information obtained justifies the efforts.In contrast, packed cell volume of suspension cultures is easily determined by pipetting a known volume into a 15 ml graduated centrifuge tube, spinning at 200 x g for 5 min and reading the volume of cell pellet, which is expressed as ml cells of culture
Culture fresh and dry weights are the most commonly used measures of growth of both suspension and callus cultures. In case of callus cultures, the cell mass is placed on a preweighed dry filter paper or nylon filter and weighed to determine fresh weight. Cells from suspension cultures are filtered onto a filter paper or nylon filter, washed with distilled water, excess water removed under vacuum and weighed alongwith the filter; the filter is preweighed in wet condition. For dry weight determination the cells and the filter are dried in an oven at 60°C for 12 hr and weighed; the filter is pre-weighed in dry condition. Cell fresh and dry weights may either be expressed as per ml (suspension culture) or per culture.