General Techniques for Tissue Culture - The technique of in vitro cultivation of plant cells and organs is primarily devoted to solve two basic problems:
(1) to keep the plant cells and organs free from microbes, and
(2) to ensure the desired development in the cells and organs by providing suitable nutrient media and other environmental conditions.
The first problem can be eliminated by using modern equipments and careful handling during various operations. The second problem remains the area of active research and is likely to do so for quite some time in the future.
At present, it relies mainly on the manipulation of culture medium, especially growth regulators, and, to a much lesser extent, of other factors, including environmental conditions.