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Laboratory Space for Tissue Culture - The organisation of a tissue culture laboratory depends mainly on the nature and the scale of activity. In general space for the following is needed:
(1) washing, drying and storage of vessels,
(2) preparation, sterilization and storage of media,
(3) aseptic handling of explants and cultures,
(4) maintenance of cultures and
(5) observation of cultures.
In a modest laboratory, provisions for activities 1 and 2 can be made in a single room (media room), while the remaining work can be done in another room (culture room). In such a situation, the following precautions should be taken.

1. The washing area should be physically separated, even by a temporary partition, from that used for medium preparation.
2. The weighing balances should be kept in a separate enclosed space.
3. Autoclave, refrigerator, deep freeze and incubators may be located in the corridor.
4. For aseptic manipulation, Laminar Flow hoods are commonly used, which can be housed in the culture room. A small table having a stereoscopic microscope may be adequate for culture observation.