Media Preparation - Media making can be time consuming. Nowadays the plant tissue culture media most commonly used are available in the market as dry powders. The simplest method of preparing media is to dissolve these powders containing inorganic and organic nutrients in some quantity of distilled water. After the contents have been thoroughly mixed in water, sugar, agar (melted), and other organic supplements are added. Finally, the volume is made up to one litre. The pH is adjusted and the medium is autoclaved. Powdered media are useful for propagation of plant species requiring nutrients according to the recipe of standard media.