Plant Tissue Culture - The conventional breeding methods are the most widely used for crop improvement. But in certain situations, these methods have to be supplemented with plant tissue culture techniques either to increase their efficiency or to be able to achieve the objective, which is not possible through the conventional methods.
The term tissue culture is commonly used in a very wide sense to include in vitro culture of plant cells, tissues as well as organs. But in a strict sense, tissue culture denotes the in vitro cultivation of plant cells in an unorganised mass, e.g., callus cultures.