Rooting of Shoots - In general, the rooting medium has low salt, e.g., 1/2 or even 1/4 salts of the MS medium, and reduced sugar levels (usually 1 g/J), reduced salts being essential for rooting in some species like Narcissus. In some species, e.g., Narcissus, strawberry, etc., rooting occurs on GR-free medium.

But in most species 0.1 -1 mg/I NAA or IBA is required for rooting. In plants like Citrus, however, a pulse treatment with an auxin (10 min with 100 mg/I NAA or IBA) gives optimum rooting.

Shoots are usually rooted in an agar medium, but the recent trend is to root them directly in vermiculite or potting mix. The cut ends of shoots are treated with a suitable auxin solution or powder mix, transplanted in pots and kept under high relative humidity and low light intensity.