Thawing of Frozen Tissue Culture Material - Thawing of the frozen materials is achieved by plunging the vials into water at 37-40°C (thawing rate of 500-750oG min-I) for 90 seconds. The material is then transferred into an ice bath till it is recultured. The thawing has to be rapid in order to avoid ice crystal formation.

Generally, the thawed material is washed several times to remove the cryoprotectant before it is recultured. But the recent trend is to reculture the material in the presence of the cryoprotectant either in diluted (more common) or undiluted state.