Transfer of Planelets to Soil,Rooted Shoots

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	Transfer of Planelets to Soil - Rooted shoots are removed from the medium, agar sticking to their roots is washed with tap water, and they are transplanted into plastic cups (ranging from ordinary to specifically design) containing a suitable potting mix. Plants are kept in high (90% or more) humidity and, initially low light intensities. High humidity can be attained by (i) fog (water drops 10 µm or less) (ii) mist or (iii) a clear plastic to cover individual (plastic bags) or groups (plastic sheets) of plants.
The potting mix should not be too wet and water drops should not form on the plantlets. For this reason, fog is preferred over mist. The humidity is gradually decreased to the ambient level after about 7-15 days, and the light intensity is increased. The plants are finally exposed to greenhouse conditions. In modest situations and on a laboratory scale, individual plants may be covered with clear plastic bags and irrigated daily with 2-3 drops of tap water or 1/4 MS salts. After 7-10 days, the bags may be removed, gradually over a period of 7-10 days.