Various
Sterilization
Techniques
Used
in
Tissue
Culture
Work
for
Different
Equipments -
| Sterilization technique |
Materials sterilized |
| Dry heat (l60-180°C for 3 hr) |
Empty glassware (culture vessels, pipettes, etc.), and certain Plasticware (Teflon FEP); instruments like scalpels, forceps, needles, etc. (glass bead sterilizers used). |
| Flame sterilization |
Instruments like scalpels, forceps, etc.; mouths of culture vessels |
| Autoclaving (121°C at 15 p.s.i.for 15-40 min) |
Media, culture vessels (glassware and several plasticware" other glass and plasticware, contaminated, cultures |
| Filter sterilization |
|
| (a) Liquid (membrane filter of 0.45 µm or smaller pore size) |
Heat labile compounds like GA3, ABA, zeatin, urea, enzymes, etc. |
| (b) Air (HEPA tilter) |
Air blown through laminar flow cabinets |
| Wiping with 70% Ethanol |
Platform of laminar flow cabinets, hands of the operator, etc. |
| Surface sterilization (using one of several sterilizing agents) |
All plant materials to be cultured |
|
