(iii) addition of agar hydrolysates, and
(iv) use of growth retardants.
Morphological variants may arise during the multiplication state. Such variants occur, in most cases, at a frequency comparable to that in vivo. But in commercial ventures using meristem cultures, visual selection is practised to eliminate variants and maintain the homogeneity of plantlets produced.
Many commercial enterprises, therefore, prefer to multiply shoots for only four or so cycles from an explant; after this, a fresh batch of cultures is initiated from field-tested plants.
|
