To increase reliability and decrease labour costs, high level of automation is also used in the process, and computer devices are used to monitor factors like pH, temperature, dissolved O2, etc. After fermentation, cell and debris are removed by centrifugation and supernatant is used for isolation of antibodies.

In recent years the need for hybridoma cells has been obviated and recombinant DNA techniques are being utilized for the production of either the whole antibodies or only a fragment thereof, which is equally useful.

The genes or parts of genes are cloned, and inserted in cells which ate cultured for the production of monoclonal antibodies.