Isolation of Tissue, Human Tissue, Animal Tissue, Alcohol, Balanced Salt Solution (BSS), Protocols, Freshney

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	Isolation of Tissue - Before working with animal or human tissue, it is essential to be sure that your work does not violate medical-ethical rules or the current legislation. You may sterilize the site with 70% alcohol and remove the tissue aseptically and transfer it to balanced salt solution (BSS) or to a suitable culture medium.
The tissue may also be stored in a refrigerator before transferring it to BSS or to a culture medium, because viable cells may be recovered from chilled tissue several days after explantation. Different protocols are available for isolation of tissues like mouse embryo, hen's egg, human biopsy material, etc. Readers are advised to consult a book on animal tissue culture to obtain protocols (e.g. Freshney, 1987).