Mechanical Disaggregation, Enzymatic Disaggregation, Cells

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	Mechanical Disaggregation -Enzymatic disaggregation is labour intensive and involves damage of cells. Therefore, mechanical disaggregation of cells is sometimes preferred. In, this method, tissue is carefully sliced and the cells which spill out are collected. Alternatively the cells are either,
(i) pressed through the sieves of gradually reduced mesh, or (ii) forced through a syringe and needle, or even (iii) repeatedly pipetted. Although the method may cause mechanical, damage, the cell suspension is more quickly obtained than in the enzymatic disaggregation. Therefore, when the availability of tissue is no limitation and the efficiency of yield unimportant, mechanical disaggregation may be used to obtain good yield of cells in a shorter time, but at the expense, of very much more tissue. Steps involved in one of the methods of mechanical disaggregation.