Separation of Viable and Nonviable Cells, Primary Cultures, Adherent Culture, Ficoll, Sodium Metrizoate, Primary Cells

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	Separation of Viable and Nonviable Cells - The dissociated cells obtained as above, usually described as primary cells, grow well when seeded on culture plates at high density. These are adherent primary cultures, but primary cultures can also be maintained in suspension. In the first case (adherent culture), nonviable cells wilt be removed at the first change of medium.
In suspension, on the other hand, non viable cells are gradually diluted out, when cell proliferation starts. However, non viable cells can also be removed from primary disaggregate by centrifuging the cells on a mixture of Ficoll and sodium metrizoate, when viable cells are collected from the interface after centrifugation.