Multienzyme Systems by Gene Fusion ( Bi and Polyfunctional Enzymes) -Multienzyme systems have been artificially synthesized, which can catalyze sequential reactions in many biotechnological production processes. Although, proximity of more than one enzymes can also be achieved by co-immobilization and chemical cross linking, gene fusion appears to have the highest potential in enzyme technology.

The gene fusion technology, for preparation of bi-and polyfunctional enzymes, involves joining of structural genes of two or more enzymes. The translational stop singal at the 3' end of the first gene is removed and ligated in frame to the A TG start codon of the second gene.