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Assessment of Somatic Hybrids and Cybrids - The nature of somatic hybrids and cybrids is assessed on the basis of morphological, cytological and isoenzyme features of the plants derived from protoplast fusion. The majority of hybrids obtained by somatic hybridization can also be obtained by sexual crossing.

Hence direct comparison can be made between the sexual and somatic hybrids and the observations used to confirm that somatic hybridization has occurred. Morphology, features of foliage, floral structure, shape, color and size are all intermediate. In addition, the hybrids" exhibit a sum of isoenzyme bands found in the parents.

Isoenzymes of constitutive enzymes used in identification of somatic hybrids include alcohol dehydrogenase, lactate dehydrogenase, esterase, glucose-6-phosphate dehydrogenase, aspartate aminotransferase, peroxidase and ribulose bisphosphate carboxylase.