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Biochemical Selection of Somatic Hybrids - Carlson et al. (1972) demonstrated the value of biochemically based selection. This selection procedure was based on a prior knowledge of the differential growth characteristics and nutritional requirements of unfused and hybrid mesophyll protoplasts isolated from the genetically different Nicotiana glauca and N. langsdorffii.

Protoplasts of the hybrid were able to grow on a defined medium in culture to form calli, whereas parental types failed to develop into calli. This selection system has an advantage in that the requirement of a mutant as one of the fusion partners is totally eliminated.

Power et al. (1976) utilized the differential sensitivity of protoplasts isolated from Petunia parodii and P. hybrida to the drug actinomycin D. In an MS medium the mesophyll protoplasts o(Petunia hybrida developed up to a macroscopic callus stage and those of P. parodii divided to form only small cell colonies.
The addition of actinomycin D to the culture medium apparently had a slight effect on the regeneration potential of P. parodii protoplasts while those of P. hybrida failed to divide.

Maliga et al. (1977) adopted a similar procedure for somatic hybrids between Nicotiana sylvestris and N. knightiana. This selection system makes use of two dominant drug resistant cell lines.
Selection of somatic hybrids following protoplast fusion of two such cell, lines is carried out on, media containing both drugs. Earlier White and Vasil (1971) regenerated hybrid cell lines from intraspecific hybridization of Nicotiana sylvestris resistant to 5-2-aminoethylcysteine and 5-methyltryptophan.

 
 

 

 
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