The culture dish is then maintained at 25-28°C. For culturing protoplasts in the nutrient medium containing agar, about 2 ml aliquots of isolated protoplasts of suitable density (ca 103-105 cells m-1) are mixed with an equal volume of agar nutrient medium, the temperature of which should not exceed 45°C. On solidification of the agar, the culture plates are sealed and maintained in an inverted position at 25-28°C.
|
