SV40 Transducting Vectors - There vectors produce viral particles after infecting monkey cells. They must have these three features:

(1) the SV 40 origin, including the surrounding region containing the transcriptional regulatory signals (i.e., regions at which splicing and polyadenylation occur),

(2) size (including, that of the DNA insert) between 3900 bp and 5300 bp for packaging into virions, and

(3) genes encoding large-T, VP1, VP2, and VP3.