Back to Home
Home >> Recombinant DNA Technology >> Comparison of PCR Versus Gene Cloning
Back to Home

Comparison of PCR Versus Gene Cloning - The PCR is a revolutionary technology.
(1) It is more efficient (as it needs much less amount of the desired DNA; a single copy is enough)
(2) does not require difficult to store and costly restriction enzymes, ligase, and vector DNA, which reduces the cost drastically;
(3) needs far less work, time and skill;
(4) and has many more applications than gene cloning. Typically, gene cloning experiments take 2-4 days, while PCR takes up to 4-5 hours. In addition,

(5) PCR is fully automated, while gene cloning is not. But for PCR one does need sequence information for construction of primers, and a thermal cycler (cost, around Rs.2 lac) or PCR machine. /t is expected that PCR will eventually take over most of the applications of gene cloning and will find many novel applications as well.