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Minichromosome Vectors - These shuttle vectors behave like very small chromosomes in that they replicate only once during each cell division and are distributed to daughter cells like true chromosomes. A typical minichromosome vector contains the following functions: from yeast,

(1) an ARS sequence (provides replication origin),

(2) a CEN sequence (regulates replication and distribution during cell division similar to those of chromosomes), and

(3) a functional gene say LEU 2, that serves as a selectable marker in appropriate yeast strains (in this case LEU2- cells); and from a bacterial plasmid,

(4) the ori or replication and

(5) a selectable marker, e.g., ampr. CEN sequences are of about 500 bp and come from the centromeric regions of yeast chromosomes; they are responsible for centromeric functions during cell division.

 

  

 
 

 
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