Ordinarily, the double stranded form is used to produce recombinant molecules since single-stranded DNAs are not cleaved by type II restriction endonucleases; this form is readily isolated from M13-infected E. coli cells.
However, the single-stranded form of M 13 is used to recover single-stranded copies of the DNA inserts; this form of vector is available from the phage particles abundant in the growth medium.
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