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Properties of M13 Vectors - M13 genome has been used to produce M13mp series of vectors, e.g., M13mp 8, M13mp 9, etc. The desirable features of M13 vectors are as follows:
(1) very large inserts can be cloned since packaging does not depend on genome size (as is the case with λ vectors).
(2) Pure single-strand copies of double-strand DNA inserts are obtained in abundance.
(3) Since DNA inserts are accepted in either of the orientations (which is also the case for plasmid and λ vectors), some recombinant clones will produce single-strand copies of one strand of the DNA double-strand, while others would produce copies of the complementary strand of the DNA insert.

The phage particles in a single plaque, as a rule, will yield copies of the same single-strand.
This property is very useful for a precise DNA sequencing (using both the strands of a DNA molecule) and for the synthesis of specific radio-labelled DNA probes.
(4) Bacterial cells infected by these vectors remain viable as in the case of plasmid vectors; this allows easy maintenance of the vector. Finally,
(5) they form plaques like λ phage vectors making selection of the recombinant DNAs rather easy, and
(6) the recombinant DNA is obtained within stable bacteriophage particles.