The phage particles in a single plaque, as a rule, will yield copies of the same single-strand. This property is very useful for a precise DNA sequencing (using both the strands of a DNA molecule) and for the synthesis of specific radio-labelled DNA probes.
(4) Bacterial cells infected by these vectors remain viable as in the case of plasmid vectors; this allows easy maintenance of the vector. Finally,
(5) they form plaques like λ phage vectors making selection of the recombinant DNAs rather easy, and (6) the recombinant DNA is obtained within stable bacteriophage particles.
|