Since the DNA inserts are generally mixtures, particularly when cDNA preparations and genomic DNA fragments are used, the various transformed clones would contain a variety of different DNA inserts.
The next step, therefore, is to identify the clone having the desired DNA insert from among the large number of clones containing the recombinant DNAs. Suitable selection strategies have been devised to achieve these two critical objectives; this is the most important step in DNA cloning.
|
