Steps in Gene Cloning,Procedure of Cloning,Recombinant DNA Technology,DNA Fragment,Transformation

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	Steps in Gene Cloning - The entire procedure of cloning or recombinant DNA technology may be classified into the following five steps for the convenience in description and on the basis of the chief activity performed. 1. Identification and isolation of the desired gene or DNA fragment to be cloned.
2. Insertion of the isolated gene in a suitable vector. 3. Introduction of this vector into a suitable organism/cell called host (transformation). 4. Selection of the transformed host cells. 5. Multiplication/expression/integration followed by expression of the introduced gene in the host.