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Yeast Vectors - Yeast, Saccharomyces cerevisiae, is an eukaryote with 34 (2n) chromosomes; it reproduces sexually as well as asexually by budding.

In suspension cultures, it grows as single cells with cell doubling time of 1.5 to 2.5 hrs, but on agar plates yeast cells produce colonies. Its haploid DNA content (1.4 x 107 bp) is only 3 times that of E. coli, and its genetics has been extensively studied. Yeast viruses are not known. Only a single yeast plasmid has been discovered, which has been used to construct some useful vectors.

The tough polysaccharide wall of yeast is an effective barrier to DNA molecules. Therefore, yeast cell wall is enzymatically digested to produce spheroplasts, which can take up DNA following treatment with CaCl2; walls regenerate in specific media. The different vectors used in yeast may be grouped as follows:

(1) plasmid vectors,
(2) ARS vectors,
(3) microchromosome vectors, and
(4) YAC (yeast artificial chromosome) vectors.

 

  

 
 

 
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