Culture
Medium -Theoretically, the medium used for raising a fast growing friable callus should prove suitable for initiating cell suspension cultures of that particular species in a liquid medium. In practice, the requirements for rapidly growing cell suspensions differ from those for tissue or callus cultures.
For example, the culture medium for a tobacco cell suspension requires an Increase in the concentration of 2, 4-D from 0.3 mg 1-1 to 2 mg 1-1, followed by supplementing the callus medium with additional vitamins and casein hydrolysate. Furthermore, the inorganic phosphate is rapidly utilized in actively growing suspension cultures and, consequently, becomes a limiting factor.
