Single Cell Culture, Plant cells, Multicellular organisms, Cell cultures, Plant biologists, Agronomic traits, Karyotype

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	Single Cell Culture - Establishment of a single cell culture provides an excellent opportunity to investigate the properties and potentialities of plant cells. Such studies contribute to our understanding of the interrelationships and complementary influences of cells in multicellular organisms.Several workers have successfully isolated single cell division and even raised complete plants from single cell cultures. Using cell cultures in studies designed to describe the pathways of cellular metabolism was another aspect that initially attracted the attention of plant biologists. It was soon realized that single cell systems have great potential for crop improvement.
Free cells in cultures permit quick administration and withdrawal of diverse chemicals and substances, thereby making them easy targets for mutant selection. Apart from this, the individual cells within a population of cultured cells invariably show cytogenetic and metabolic variations depending on the stage of the growth cycle and culture conditions. Such variability, termed spatial heterogeneity, has been the subject of much interest since differences between cells in their karyotype and the ability to accumulate secondary metabolites are manifested during morphogenesis in the clones regenerated from single cells. In this way the cell line selection technique can be usefully applied to produce high yielding cultures as well as plants with superior agronomic traits.