The incubation period from culture initiation to the stationary phase is determined primarily by:
a) initial cell density,
b) duration of lag phase, and
c) growth rate of cell line.
While initiating a new suspension culture it is necessary to determine optimal cell density, proportionate to the volume of the culture medium, in order to achieve maximum growth.
Cell cultures initiated at very low densities will not grow unless the medium is enriched with the metabolites necessary to grow single cells or a small population of cells.
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