Embryo Culture - For embryo culture, embryos are excised from immature seeds, usually under a hood (laminar flow cabinet), which provides a clean aseptic and sterile area. Sometimes, the immature seeds are surface sterilized and so soaked in water for a few hours, before the embryos are excised. The excised embryos are directly transferred to a culture dish or culture tube containing synthetic nutrient medium.

The entire operation is carried out in the laminar flow cabinet and the culture plates or culture tubes with excised embryos are transferred to a culture room maintained at a suitable temperature, photoperiod and humidity.

(iii) to raise synthetic crops like triticale by producing amphiploids from the hybrids that the distant hybrids which can not be obtained even through embryo culture, can be obtained through protoplast fusion.

Embryo culture has also been used for haploid production through distant hybridization followed by elimination of the chromosomes of one parent in the hybrid embryos cultured as above.

A popular example includes hybridization of barley and wheat with Hordeum bulbosum leading to the production of haploid barley and haploid wheat respectively. Haploid wheat plants have also been successfully obtained through culture of hybrid embryos from wheat x maize crosses.