Endosperm
Culture - Tissue culture methods are also used for culturing endosperm, which is unique firstly, in its function of supplying nutrition to the developing embryo and secondly, .in being triploid in its chromosome constitution. Triploid plants are useful for production of seedless fruits (e.g. apple, banana, watermelon, etc.) and for production of trisomics for cytogenetic studies.
Generally, these triploids are routinely obtained by crossing colchicine induced tetraploids with diploids followed by rescuing the triploid embryos. However, there may be strong crossability barriers, making it difficult to produce triploids from 4x X 2r crosses, so that endosperm culture may be used as an alternative method for triploid production.
