Culture of Adult Organs - The culture of organs or parts from adult animal is more difficult due to their greater requirement of O2' A variety of adult organs (e.g. liver) have been cultured using special media (e.g. T8; see Paul, 1975) with a special apparatus (Towell's Type II culture chamber).

Since serum was found to be toxic, serum free media were used, and the special apparatus permitted the use of 95% O2.