Flask CulturesPolystyrene Culture Flasks, Thick Clot Cultures, Thin Cultures

www.molecular-plant-biotechnology.info

Home >> Tissue and Organ Culture >>Tissue and Organ Culture

	Flask Cultures - The main use of flask cultures is in the establishment of a strain from fresh explants of tissue. A good Carrel flask has excellent optical properties for microscopic examination, even though polystyrene culture flasks can also be used, provided they have a wide neck for handling the explants. The flask technique has the following advantages:
(i) tissue can be maintained in the same flask for months or even years; (ii) large number of cultures can be easily prepared and large amount of tissue can be grown with large amount of medium. There are two types of flask techniques (i) 'thick clot cultures', which allow rapid growth suitable for short term cultures and (ii) 'thin clot cultures', which can be maintained for a considerable period of time.