Patching the Plasma Clot in Slide Cultures - If there is evidence of liquefaction, plasma clot should be patched as follows:

(i) wash small coverslip with culture as above;

(ii) in a watch glass take two drops of a mixture of plasma and BSS and to this add two drops of a mixture of serum and embryo extract; mix this quickly and place a drop on each washed coverslip having a culture;

(iii) a clean depression slide with petroleum jelly is then attached, as usual.