Preparation of Flask Cultures, Plasma Clots, Explants

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	Preparation of Flask Cultures - Following steps are involved in the preparation of flask cultures: (i) place upto six D3.5 Carrel Flasks in a rack with their necks flamed and pointing to the right; (ii) place a drop of plasma on the floor of flask and spread this plasma out in a circle;
iii) with the help of spatula, transfer the desired number of explants to the plasma and allow clotting to occur; iv) after the plasma clots and explants fixed in position, add extra medium; for thick clots 1.2ml of dilute plasma and for thin clots 1.2ml of dilute serum is added instead of plasma; the whole thing is left for clotting; (v) the flasks are gassed with gas phase (5% CO2 in air).