Test Tube Cultures - Test tubes are cheap and convenient vessels for tissue culture and can be used for preparing a large number of cultures, which can be placed in stationary racks or roller drums.

However, this technique has disadvantages like poor optical property for microscopy, difficulty in quantitation due to the curvature and high risk of contamination.

Cultures on plasma clots in test tube are prepared just like those in flasks, but tissues may be grown on the wall of test tube without a plasma clot. Test tubes may also be used for developing suspension cultures.The feeding, patching and transfer of culture is done as in other primary explantation techniques.