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Tissue and Organ Culture - We described the culture media, culture methods and the development of primary cultures and cell lines in animals. For the development of primary cultures and cell lines, a variety of tissues and disaggregation methods are used to give good yield of separate cells.

H is, therefore, obvious that for any exercise involving animal cell and tissue culture, tissue needs to be obtained under aseptic and sterile conditions, although it mayor may not be cultured, depending upon whether or not it is immediately subjected to disaggregation to give an yield of cells.

However, even for obtaining cell cultures, often the tissue is cultured using primary explanation technique developed by Harrison (1907), Carrel (1912) and others. This technique is used for cultivation of pieces of fresh tissue derived from the organism, and this was almost the exclusive technique used for animal tissue culture till about 1945. Different forms of primary explanation techniques are still widely used and will continue to be used for a very long time.

These techniques differ only in the type of vessels (flasks, test tubes. etc.) used for growing the tissue, but are uniform in principle. The primary explanation technique is also used for embryo and organ culture, but is variously modified to become specialized techniques.

The different explanation techniques are classified into the following:

i) slide cultures,

(ii) carrel flask cultures and

(iii) roller test tube cultures.

These three techniques of tissue culture and the techniques of organ culture (including tissue engineering) will be discussed in this chapter.

 
 

 
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