Transcription OR Gene Expression - The presence of a gene in the genome is not sufficient. It should express or it should make protein, only then does it have some significance or use for the organism. Genes make proteins by a process called gene expression. Gene expression takes place in two steps or phases.

1. Transcription or RNA synthesis
2. Translation or protein synthesis

Transcription is the first step in gene expression and the predominant level of gene regulation. Transcription is a process in which one type of nucleic acid produces another type of nucleic acid or it is the process of RNA synthesis from DNA molecule with the help of complex protein.

1. Ribonucleotides are used instead of DNA.

2. T is replaced with U.

3. A primer is not involved.

4. Only a small portion of DNA is used.

5. No proofreading activity.

The sequence of ribonucleotides in an RNA molecule is complementary to the sequence of deoxyribonucleotides in one strand of the double stranded DNA molecule. The strand that is transcribed into an RNA molecule is referred to as the template strand of the DNA. The other DNA strand is frequently referred to as the coding strand.