Analysis of Transgene Integration - The integration of transgene into the genome is confirmed by Southern hybridization of genomic DNA extracted from putative transgenic individuals; the DNA is digested with a suitable restriction enzyme prior to electrophoresis.

By choosing appropriate restriction enzymes for DNA digestion, not only the integration of trans gene can be established beyond doubt, but information on the number of copies per cell, the orientations of tandemly arranged copies and the presence of single or multiple integration sites is also obtained from Southern hybridization. All the individuals that give positive result with Southern hybridization are regarded as confirmed transgenics.