| Vector |
Derived from |
Features |
| 1. SV40 vectors |
|
|
| (a) Early region replacement vectors |
Replacement of large-T gene of SV40 |
1. Produce virions, which infect host cells
2. Transient gene expression
3. Mammalian cells are hosts of SV40 |
| (b) Late region replacement vectors |
Replacement of VPI, VP2, and VP3 genes of SV40, e.g., SVGT-5 |
| (c) Plasmid vectors |
Origin of replication and large-T gene of SV40 |
-- |
| (d) Shuttle plasmid vectors |
Plasmid vector [item l(c)] plus pBR322 origin and amp' gene, e.g., pSV2, pSV3, etc. |
-- |
| |
Rous sarcoma virus promoter in place of SV40 early promoter, e.g.,pRSV. |
Strong expression of the marker gene |
| (e) Passive transfecting vectors |
SV40 transcription regulatory and polyadenylation sequences, plus pBR322 origin and amp gene |
Shuttle vectors; used for gene integration in mammalian cells |
| 2. BPV vectors
|
Bovine papilloma virus "Transforming region" + pBR322 sequences. |
Shuttle vectors; often pBR322 sequence deleted prior to transfection; plasmid-like vector |
| 3. Retrovirus vectors |
pBR322 + retrovirus sequences |
Shuttle vectors; integrates as provirus into mammalian genome; produces virions |
| 4. Polyomavirus vectors |
Polyoma virus origin and early region + pBR322 sequences |
Similar to SV4O vectors; mouse cells used as host |
| 5. Vaccinia virus |
DNA insert placed within the thymidine kinase gene of virus by a process of recombination. |
|
| 6. P element vectors |
Drosophila transposable element P; minimum of 31 bp inverted repeat borders and the neighbouring regions, plus an E. coli vector, e.g.. pUC8; DNA insert of up to 40 kb placed within the two borders. |
|
| 7. Bacculovirus vectors |
Nuclear polyhedroma virus (NPV) polyhedrin gene replaced by DNA insert; e.g., AcNPV (Autographa californica nuclear polyhedroma virus) and BmNPV (Bombyx mori nuclear polyhedroma virus) vectors |
|
