Transfection
Methods - Several
approaches
have
been
used
for
the
introduction
of
DNA
into
animal
cells/embryos,
which
are
listed,
as
follows:
(i)
calcium
phosphate
precipitation,
(ii)
direct
micro
injection,
(iii)
retrovirus
infection,
(iv)
lipofection,
(v)
particle
gun
delivery
and
(vi)
electroporation.
In
addition,
transgenic
mice
are
readily
produced
by
the
technique
of
embryonic
stem
(ES)
cell
transfer;
this
is
the
only
approach,
which
permits
targetted
gene
transfers.
