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Transgenic Animal Production - The ease with which transgenic animals can be produced and the transfection techniques to be employed depend chiefly on the reproductive biology, husbandry requirements and responses to various experimental procedures of the concerned animal. The following features greatly facilitate gene transfer.

1. Production of larger number of eggs either naturally or in response to hormone regimens used for superovulation.

2. Short breeding cycle, greatly facilitates analysis of the transgenic individuals produced.

3. It is desirable that ovulation occurs throughout the year so that ova are readily available for experimentation.

4. The size and the structure of eggs should be amenable to microinjection.

5. In many animal species, body size may be an important factor to allow sufficient tissue samples to be taken for the detection of transgene integration and function.

6. In case of mammals, where transfected ova/embryos must be transferred into surrogate mothers, the following features are critical