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Cauliflower Mosiac Virus (CaMV) -

CaMV contains about 8 kb double-strand DNA genome and produces Spherical particles. CaMV infections are systemic, and even its DNA is infectious when inoculated on abraded plant surfaces.

CaMV genome has 8 tightly packed genes, of which only two small genes, genes II and VII, are nonessential; as a result, only these two genes can be replaced/deleted without a loss of infectivity. In addition, CaMV genomes exceeding the natural genome size (8024 bp) by even a few hundred bp are not packaged into virions.

These two features seriously limit the size of DNA insert clonable in CaMV. Bacterial dhfr (dihydrofolate reductase) gene was inserted in the CaMV genome in place of gene II, and was successfully expressed in plants.