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Comparison of Some of The Important DNA  Delivery Techniques For Plant Cells -

  DNA delivery method
Feature Agrobacterium-mediated Chemical-electrical Microinjection Particle gun
Size of DNA construct < 50 kb 5-and20 kb 16 kb -
Range of plants Limited All plants All plants All plants
Efficiency High High - -
DNA insert integrity Not affected by rearrangements High degree of rearrangements - High degree of rearrangements
Need for special equipment No No (chemical)
Yes (eletrical) 		Yes Yes
Regeneration protocol Needed Needed Needed(often) Not necessary
Protoplast culture Not needed Necessary May be needed Not needed
Applicable to organised meristems Generally,No No No Yes
Applicable to cereals Applicable Frequently used Possible High applicability
Chimaeric plants No No No Often


 

  

 
 

 
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