| Vector type |
Sequences present |
Derived by |
Used in Transformation |
Example |
| Cointegrate vector |
1. Single plasmid |
Homologous recombination between an IV (used for cloning the DNA insert in E. coli) and a pTi (ordinarily disarmed) |
Used alone since it has both vir region and T-DNA borders |
pGV2260 |
| |
2. pTi sequences: virregion, at least T-DNA borders and origin of DNA replication + anE. coli plasmid, e.g., pBR 322, and some T-DNA sequences, including the DNA insert, placed within T-DNA (without borders). |
|
|
| Binary vector |
1. A pair of plasmids; one contains vir region, and the other has T-DNA |
Mini Ti produced by integrating pTi |
Mini-Ti and 'helper' pTi must be used together; both the plasmids must be present in the same Agrobacterium cell. |
Bin19 (Mini-Ti) and pAL 4404 (helper Ti) |
| |
2. One plasmid (called mini-Ti or micro-Ti is an E. coli plasmid, e.g., pRK252, which contains disarmed T-DNA; used for cloning of DNA insert in E. coli |
T-DNA into an E. coli plasmid |
|
|
| |
3. A 'helper' pTi, which contains the vir region, but ordinarily lacks T-DNA (including the border sequences) |
Helper pTi obtained by deletion of T-DNA sequences from pTi |
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