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Golden Rice - Vitamin A-deficiency causes blindness among children and may even lead to their death. Vit. A-deficiency often occurs where rice is the staple food, since rice grain does not contain provitamin A, i.e., β-carotene.

Three transgenes providing phytoene synthase, phytoene desaturase, zeta-carotene desaturase and lycopene cyclase activities were transferred into rice by Agrobacterium mediated transformation. All the trans genes were introduced together in a single co-transformation experiment.

The resulting transgenic rice, popularly called "golden rice" contains good quantities of β-carotene, which gives the grains a golden colour. In one transgenic line the β-carotene content was as high as 85% of the total carotenoids present in the grain.

Iron-deficiency anaemia is the most common nutritional disorder in the world. Rice grain has the lowest iron content among crops; it also has phytate, which reduces iron absorption in human intestine by up to 98%. In addition, iron absorption from a vegetarian diet is rather poor.ate on the effects of developments in biotechnology on the environment.

Phytate is used for phosphate storage in seeds, and it is used during seed germination. The high iron rice was produced expressing in rice three transgenes, viz ferretin encoding gene metallothionein gene and a thermostable phytase encoding gene.

A 'high iron-high provitamin A' rice line was produced by crossing the 'golden rice' and 'high iron' transgenic lines of rice. It is proposed t9 distribute this rice line free to subsistence farmers of the developing world with a view to alleviate vitamin A and iron deficiencies.