Important Transgenes Which Modify Starch Lipid or Produce a Novel Non Protein

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Important Transgenes Which Modify Starch Lipid or Produce a Novel Non Protein -

Gene encoding the Enzyme	Source	Expressed in	Consequences of expression
Starch
Granuule bound starch synthase (cDNA antisense construct)	Potato	Potato	1. Little or no amylase synthesis; 2.Starch yields clear paste, which does not deteriorate 3. Useful in preparation of microwave ready foods
Cyclodextrin glycosyltransferase	Klebsiella pneumoniae	Potato	α - β- cyclodextrins produced
ADP-glucose pyro-phosphorylase	E.coli (gene glgC16)	Potato (llow starch lines)	1. 60% more starch than control
Fructosyl transferase	Bacillus subtilis	Potato	1.Fructan up to 1-30% of leaf dry wt., and up to 1-7% of microtuber dry wt. 2. Total nonstructural carbohydrates in leaves upto 35% as compared to only 7% in the control
Lipids
Stearoyl-ACP desaturase (antisense construct)	Brassica campestris	B. rapa and B. napus	1. Increased stearic acid (from < 2% to up to 40%) 2. Decreased oleic acid content
Thioesterase	California bay tree	Arabidopsis thaliana	1. Accumulation of 12-carbon fatty acids
Biodegradable plastic
1. Acetyl-CoA reductase (phbB),	Alcaligenes eutrophus	Arabidopsis thaliana	1.Polyhydrobutyrate accumulated in plastids
2. PHB synthase (phbC)			2. Up to 10 mg/g fresh wt. PHB accumulated when the enzymes were targetted into plastids