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Integartion of Transgenes -

The major part of DNA introduced into plant cells by direct DNA delivery methods is degraded, and only a small portion of it becomes integrated into the plant genome. The vector DNA may become rearranged, deleted or become joined end-to-end to form tandem repeats.

Different unlinked DNAs introduced together become linked together and are ordinarily co-transformed. Many of the features of transgene integration into plant cells are comparable to those in animal cells. Some of the main features of transgene integration from direct DNA delivery into the plant cell genome are listed below.

1. Integration occurs at random sites in the genome.
2. Linearized DNA is much more effective in producing stable integrations than circular DNA.
3. Different unlinked DNAs introduced together usually become linked in the cell and show co-transformation:
4. Multicopy integrations usually occur in tandem at one site.
5. Higher DNA concentrations increase the integration frequency but number of copies also becomes high (5-10).
6. Use of carrier DNA in some of the direct DNA delivery techniques enhances the frequency of stable integration. But it also promotes multicopy integrations and complex gene rearrangements.

 

  

 
 

 
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